HepG2 cell line is derived from a human hepatocellular carcinoma line (We et. al., 2006). Hepatoblastoma (HB) or hepatocellular carcinoma is an embryonal malignancy of hepatocellular origin and the most common primary liver tumour of childhood, usually presenting in the first year of life.
Before we go any further, let us explore about what is cancer cell line first. Cancer cell lines are widely used as experimental models of neoplastic disease, and data interpretation depends on unambiguous attribution of the respective cell line to its original source (Walther et. al., 2005). The progress of cell culture techniques enable the researchers to establish varieties of human carcinoma cell lines from surgical and autopsy tissues, peritoneum effusion, biopsy specimens and so on. As the pure cells in cell cultures can be applied for many types of researches that cannot be performed using tissue specimens, the research of permanent cell lines established form human cancers plays an important contributing role to help us understand the biology of cancers (Ku et. al., 2002).
HepG2 – Liver Cancer Cell Line
Let us go back to talk about HepG2 cell line. HepG2 cell line was introduced since 1979 (Norman et. al., 1992). Until now, there are more than nine thousands HepG2 references found in the scientific literature. This cell line has been proven to be a useful model of the human liver cell as there is high proportion of liver-specific proteins identified in the medium, for which there are antisera commercially available (Liu et. al., 1985). The metabolic pathways that have been studied appear to simulate the behaviour of normal hepatocytes. Thus, the studies encourage a wider application of this HepG2 cell line to biological problems that relate specifically to the role of the liver (Norman et. al., 1992).
Therefore, HepG2 cell line has been widely used in varieties of fields like liver metabolism, development, oncogenesis and hepatoxicity (Dolores et. al., 2009).
In conclusion, HepG2 cell line is an useful cell line that can be used in variuos field of cancer research. I used it for my final year project too. 🙂
This is a part of the literature review of my final year project thesis. I wish to share with you all what i have done on it.